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Wolgang Arnold   Professor  Senior Scientist or Principal Investigator 
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Wolgang Arnold published an article in March 2019.
Top co-authors See all
Oliver Schildgen

424 shared publications

Kliniken der Stadt Köln gGmbH, Klinikum der Privaten Universität Witten Herdecke, Ostmerheimer Str. 200, D-51109 Cologne, Germany

Thomas Dittmar

35 shared publications

Department of Immunology, Center for Biomedical Education and Research, Faculty of Health, Witten/Herdecke University, 58453 Witten, Germany

Peter Gaengler

27 shared publications

ORMED Institute for Oral Medicine at the University of Witten/Herdecke, Witten, Germany

Gh. Danesh

19 shared publications

Department of Orthodontics, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany

Rainer A Jordan

18 shared publications

Department of Operative and Preventive Dentistry, Faculty of Health, School of Dentistry, Witten/Herdecke University, Witten, Germany

Publication Record
Distribution of Articles published per year 
(2002 - 2019)
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Article 1 Read 0 Citations Facial fold and crease development: A new morphological approach and classification Tudor Sandulescu, Marie Franzmann, Julia Jast, Tania Blauroc... Published: 07 March 2019
Clinical Anatomy, doi: 10.1002/ca.23355
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Objective Facial folds and creases are established descriptive anatomical terms for structures of which the morphological characteristics and origins are not clearly defined. The aim of this study was to perform a morphological investigation of the nasolabial fold (NLF), mandibular fold (MF), deep transverse forehead (DTFC), infraorbital fold (IOF) and upper eyelid fold (UEF), correlating their phenotypes to differences in the superficial musculoaponeurotic system (SMAS), noting morphological differences and similarities. Methods Full‐graft tissue blocks of skin, subcutaneous tissue and mimic muscles collected postmortem were studied histologically. Serial histological sections were stained with Azan. Location‐ and composition‐specific morphological differences were determined. Histological serial section digitalization and three‐dimensional reconstruction of the tissue blocks were performed. Results Three different types of SMAS architecture were identified. Type I SMAS consisted of parallel‐aligned fibrous septa connecting the mimic muscles to the skin that covered the cheek, infra‐ and supra‐orbital and forehead areas. Type II SMAS morphology appeared as a condensed type I SMAS architecture with stronger fibrous septa and smaller fatty tissue compartments covering the lower and upper lip areas. Type III SMAS consisted of loose connective tissue covering the lower and upper eyelid regions. Conclusions NLF, MF, IOF and UEF are habitual primary folds induced by morphological changes in the underlying SMAS architecture. The secondary, accidental creases (DTFC) are cutaneous depressions derived from interacting dermal‐skeletal‐muscular changes without SMAS structure changes. The upper and lower eyelid wrinkles were tertiary, age‐related undulating skin redundancy formations. This article is protected by copyright. All rights reserved.
Article 0 Reads 0 Citations Histological, SEM and three-dimensional analysis of the midfacial SMAS — New morphological insights T. Sandulescu, H. Buechner, D. Rauscher, E.A Naumova, W.-H A... Published: 01 March 2019
Annals of Anatomy - Anatomischer Anzeiger, doi: 10.1016/j.aanat.2018.11.004
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The superficial musculoaponeurotic system (SMAS) of the midface has a complex morphological architecture, and a multitude of controversial opinions exist regarding its in vitro appearance and clinical relevance. The aim of this study was to investigate the three-dimensional architecture of the midfacial SMAS. Histological and SEM analyses were performed on tissue blocks of the skin, subcutaneous tissue and mimic musculature of the midfacial region between the anterior parotid gland pole and lateral to the nasolabial fold and tissue blocks of the skin, subcutaneous tissue and parotid fascia. Blocks were collected postmortem from six formalin-fixed donor bodies. Serial histological sections were made, stained with Azan and digitized. Three-dimensional reconstructions and visualization of the tissue blocks were performed using AutoCAD. Two different SMAS architectures were found in the midfacial region: parotideal (type IV) and preparotideal (type I) SMAS. Type I SMAS showed three-dimensional interconnecting fibrous chambers embracing fat tissue lobules that cushioned the space between the skin and mimic musculature. Fibrous septa divided the mimic musculature surrounding the muscular bundles. Beneath the mimic muscular level, SMAS septa were oriented parallel to the muscular plane. Above the mimic muscular plane, SMAS septa were oriented perpendicularly, inserted into the skin. Type IV SMAS showed a parallel alignment of the fibrous septa to the skin level, anchoring the skin to the parotid fascia, presenting lymphatic nodes in the fat tissue compartments. The fat cells of the SMAS were enveloped in a fibrotic membrane at the border of the fibro-muscular septa. The SMAS blood supply comprised two subcutaneously epimuscularly spreading anastomosing vascular systems. Midfacial SMAS represents a functional unit with physical and immunological tasks appearing in two different morphological architecture types. A well-defined nomenclature is needed to prevent controversy.
Article 0 Reads 0 Citations Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity Ella A. Naumova, Alexander-Simon Engel, Hagen Tizian Kranz, ... Published: 06 February 2019
Coatings, doi: 10.3390/coatings9020099
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Bacterial biofilms adhere to all oral surfaces and may alter or degrade them. For investigations of the oral biofilm, growing on new restorative dental biomaterials, sterilized dental enamel surfaces as natural, control, and reference materials are used. A novel method for disinfection and sterilization of surfaces is low-pressure plasma (LPP) sterilization, which is a nondestructive and nontoxic technology. The roughness of the dental enamel surface was determined before and after LPP sterilization. Enamel discs were placed in dental splints and worn for five days in vivo. Oral biofilm was fixed for scanning electron microscopy (SEM). Biofilms growing in vitro were characterized microbiologically before and after sterilization and examined by confocal laser scanning microscopy (CLSM). Microbiology demonstrated that various bacterial strains were present in the biofilms. SEM showed multiple layers of densely packed bacteria, and CLSM demonstrated that the biofilm contained live and dead bacteria. After LPP sterilization, no biofilm could be detected, and the enamel surface remained unaltered. It may be concluded that LPP sterilization is an effective, nondestructive method for disinfection of enamel before application in the oral cavity. LPP sterilization may be suitable for sterilization of dental materials without altering their surfaces.
Article 1 Read 1 Citation Bacterial viability in oral biofilm after tooth brushing with amine fluoride or sodium fluoride E.A. Naumova, L. Weber, V. Pankratz, V. Czenskowski, W.H. Ar... Published: 01 January 2019
Archives of Oral Biology, doi: 10.1016/j.archoralbio.2018.10.013
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The aim of this study was to investigate the effects of sodium fluoride (NaF) and amine fluoride (AmF) on bacterial viability in the oral cavity. Healthy subjects brushed their teeth with either fluoride free toothpaste, NaF- or AmF-containing toothpaste. Biofilm smears from different locations were collected before and immediately and 30 and 120 minutes after tooth brushing. The smears were stained with live/dead bacterial staining, and the number of the respective bacteria was counted. The data were statistically analyzed by comparing the numbers of bacteria before and after the application of no fluoride, NaF and AmF. The highest numbers of bacteria were found in the tongue biofilm, followed by the palatal and cheek biofilm. The lowest numbers were found in the mouth floor biofilm. After the application of AmF, no changes in the numbers of bacteria were found in the biofilms, except for the cheek, where they were reduced. After the application of NaF, the number of bacteria decreased significantly in all biofilms. After 120 minutes, bacterial regrowth was complete. AmF has only little effect on the bacterial viability of oral biofilms. NaF application reduces the number of living bacteria in the oral biofilms. This effect lasts not longer than 120 minutes.
Article 0 Reads 0 Citations Human Bocavirus Infection of Permanent Cells Differentiated to Air-Liquid Interface Cultures Activates Transcription of ... Verena Schildgen, Monika Pieper, Soumaya Khalfaoui, Wolfgang... Published: 30 October 2018
Cancers, doi: 10.3390/cancers10110410
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The parvoviral human bocavirus (HBoV) is a respiratory pathogen, able to persist in infected cells. The viral DNA has been identified in colorectal and lung tumors and thus it was postulated that the virus could be associated with tumorigenesis. This assumption was supported by the fact that in HBoV-infected patients and in an in vitro cell culture system, pro-cancerogenic and -fibrotic cytokines were expressed. In this work, it is shown by a whole transcriptome analysis that, also at the mRNA level, several pathways leading to neoplasia and tumorigenesis are significantly upregulated. In total, a set of 54 transcripts are specifically regulated by HBoV, of which the majority affects canonical pathways that may lead to tumor development if they become deregulated. Moreover, pathways leading to necrosis, apoptosis and cell death are downregulated, supporting the hypothesis that HBoV might contribute to the development of some kinds of cancer.
Article 2 Reads 0 Citations Influence of Luting Materials on the Retention of Cemented Implant-Supported Crowns: An In Vitro Study Ella A. Naumova, Felix Roth, Berit Geis, Christine Baulig, W... Published: 28 September 2018
Materials, doi: 10.3390/ma11101853
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The retention force of cemented crowns on implant abutments with various luting materials was evaluated. Cobalt–chromium crowns were cemented onto tapered titanium abutments (Camlog) with eugenol-free temporary cement (RelyX TempBond NE), composite-based temporary cement (Bifix Temp), zinc phosphate cement (Harvard Cement), glass-ionomer cements (Meron, Fuji I), and resin-modified glass-ionomer cements (Fuji II, Fuji Plus, Ketac Cem Plus). Specimen aging via hydrostress was performed in artificial saliva at 37 °C for 14 days (S1), followed by hydrothermal stress with thermocycling (S2). The crowns were removed, and the force was recorded (T1). Subsequently, the crowns were recemented, aged, and removed, and the force was recorded (T2, T3). The retention forces differences were statistically significant according to the storage conditions at T1 (p = 0.002) and T3 (p = 0.0002). After aging (S1), Ketac Cem Plus had the highest retention force median value difference (T3 versus T1) (−773 N), whereas RelyX TempBond NE had the lowest (−146 N). After aging (S2), Meron had the highest retention force median value difference (−783 N), whereas RelyX TempBond NE had the lowest (−168 N). Recementation decreased the retention force of the implant-supported cobalt–chromium crowns cemented and recemented with the same luting materials. Luting materials (at T1) and aging conditions significantly impacted the retention force.